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For LB plates, add 15 g of agar. plate on LB with 100 µg/ml ampicillin and incubate overnight. to a final concentration of 5 µg/ml. Incubate 10 minutes at.Search results for kanamycin at Sigma-Aldrich. ADVANCED SEARCH; STRUCTURE SEARCH; CERT OF ANALYSIS; SDS SEARCH; Sigma-Aldrich ®. LB Agar Kanamycin-50, Plates.

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. DH10B and JM109) were picked from LB-agar + ampicillin plates and resuspended in PCR grade water. 1 µl resuspended bacterial cells was used as template in a 25.

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