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- Transformation with pBNAN - biobuilderjunior.orgLB Agar Kanamycin-50, Plates - Sigma-Aldrich. LB Agar Kanamycin-50,. Bacterial Culture Media,. Kanamycin A. working concentration,.be easily detected on X-Gal indicator plates. produces white (LACI+) colonies on LB plates containing ampicillin (100 µg/ml) and X-Gal. agar plate Count blue.
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ampicillin (100 µg/ml) and chloramphenicol (12.5 µg/ml). 108 LB agar indicator plates containing Fosmid Autoinduction Solution (Epicentre, Madison, USA) for.
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Blackwell Publishing, Ltd. Genomic tools and cDNA derivedGenomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white screening and 0.1% ampicillin,.1 Genomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white 116 screening and 0.1% ampicillin,.Plasmids are generally prepared from bacterial cultures grown in the presence of a selective agent such as an antibiotic. The yield and quality of the plasmid DNA.
SUPPLEMENTAL MATERIAL - hal.inserm.frOpen Archive Toulouse Archive. (LB) plates supplemented with ampicillin. antifungal activities determination, Fusarium sp. was grown in potato dextrose agar.
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by use of high saline concentration or capture by. then selected on plates of LB containing 100mg/ml. (selected on LB Agar containing 100mg/ml ampicillin).
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pGOODs: new plasmids for the co-expression of proteins in. bovine manure with a concentration of 30 t per ha. (LB) agar plates and used to inoculate 96-well. containing one ml LB 2X broth with ampicillin (50 µg.. (in micrograms per milliliter): for E. coli, ampicillin. on Pseudomonas isolation agar plates. from agar plates and suspended in LB.
For LB plates, add 15 g of agar. plate on LB with 100 µg/ml ampicillin and incubate overnight. to a final concentration of 5 µg/ml. Incubate 10 minutes at.Search results for kanamycin at Sigma-Aldrich. ADVANCED SEARCH; STRUCTURE SEARCH; CERT OF ANALYSIS; SDS SEARCH; Sigma-Aldrich ®. LB Agar Kanamycin-50, Plates.
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Gibson Assembly® HiFi 1 Step Kit | VWRInteractions with M Cells and Macrophages as Key. Cells and Macrophages as Key Steps in the Pathogenesis of. onto LB agar plates (BD.
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Mix for PCR on difficult templates : KAPA2G Robust PCR. DH10B and JM109) were picked from LB-agar + ampicillin plates and resuspended in PCR grade water. 1 µl resuspended bacterial cells was used as template in a 25.
The plate was then briefly agitated and read using the Spectra Max M2 microplate. and the cells plated on LB-agar plates containing ampicillin,.