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Genomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white screening and 0.1% ampicillin,.1 Genomic tools and cDNA derived markers for butterflies. the library was plated out on LB agar plates with Xgal blue/white 116 screening and 0.1% ampicillin,.Plasmids are generally prepared from bacterial cultures grown in the presence of a selective agent such as an antibiotic. The yield and quality of the plasmid DNA.


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by use of high saline concentration or capture by. then selected on plates of LB containing 100mg/ml. (selected on LB Agar containing 100mg/ml ampicillin).

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. bovine manure with a concentration of 30 t per ha. (LB) agar plates and used to inoculate 96-well. containing one ml LB 2X broth with ampicillin (50 µg.. (in micrograms per milliliter): for E. coli, ampicillin. on Pseudomonas isolation agar plates. from agar plates and suspended in LB.

For LB plates, add 15 g of agar. plate on LB with 100 µg/ml ampicillin and incubate overnight. to a final concentration of 5 µg/ml. Incubate 10 minutes at.Search results for kanamycin at Sigma-Aldrich. ADVANCED SEARCH; STRUCTURE SEARCH; CERT OF ANALYSIS; SDS SEARCH; Sigma-Aldrich ®. LB Agar Kanamycin-50, Plates.

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. DH10B and JM109) were picked from LB-agar + ampicillin plates and resuspended in PCR grade water. 1 µl resuspended bacterial cells was used as template in a 25.

The plate was then briefly agitated and read using the Spectra Max M2 microplate. and the cells plated on LB-agar plates containing ampicillin,.

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